Coding

Part:BBa_K2963018:Design

Designed by: Experiment group members of JNU-China   Group: iGEM19_JNU-China   (2019-09-12)


pgsB - encoding a subunit of poly-γ-glutamic acid synthetase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1032
    Illegal PstI site found at 1013
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1013
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1032
    Illegal PstI site found at 1013
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1032
    Illegal PstI site found at 1013
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When we express the PgsC protein heterologously in corynebacterium glutamicum, we add Ptac promoter and a short RBS, which can be recognized by corynebacterium glutamicum. And simultaneously, we add T7 terminater used for stopping transcription in time.


Source

Derived from Bacillus subtilis.

References